Characterization of Pajaroellobacter abortibovis, the etiologic agent of epizootic bovine abortion. Brooks RS(1), Blanchard MT(1), Clothier. J Vet Diagn Invest. May;14(3) Diagnosis of epizootic bovine abortion in Nevada and identification of the vector. Hall MR(1), Hanks D, Kvasnicka W. Kennedy PC, Casaro AP, Kimsey PB, Bon Durant RH, Bushnell RB, Mitchell GM. The development of the fetal lesions of epizootic bovine abortion (EBA) was.

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Flow cytometric analysis identified the presence of P. In light of these data, this bacterium was chosen as a primary candidate for the putative agent of EBA. Experimentally induced immunity to chlamydial abortion of cattle. The phylogenetic position of SerratiaButtiauxella and some other genera of the family Enterobacteriaceae. Intracellular bacteria of porcine proliferative enteropathy: The early changes observed in the fetuses consisted of transformation and proliferation of lymphocytes and mononuclear phagocytes.

Inoculation with negative control necropsy tissue. Thymic lesions unique to EBA include a loss of cortical thymocytes and extensive infiltration of macrophages into the medulla. Initial results using the small panel of nine bovine tissues see above showed complete concordance with the EBA status of the animals. Immunofluorescent examination demonstrated that immunoglobulins Ig G and IgM were present in the vascular lesions.

Argasidae with the exposure of cattle to epizootic bovine abortion in California.

These lesions were similar to immune-mediated lesions that result from the deposition of toxic complexes in the tissues. This approach was used because preliminary cloning experiments using universal bacterial primers yielded sequences identical to those of Pseudomonas sp.

The sequential development of the fetal lesions was studied and the lesions compared with those in both naturally occurring and experimentally induced infections of the dams which either aborted or carried to term.


Polymerase chain reaction for detection of Borrelia coriaceaeputative agent of epizootic bovine abortion.

A combination of direct epixootic bp from 25 EBA-positive fetuses representing 16 field cases, four experimental tick transmissions, and five experimental tissue transmissions, the last using thymus homogenate derived from an EBA-positive fetus and Southern blotting of these amplicons was used to confirm PCR specificity.

Epizootic bovine abortion characterized by foetal hepatopathy. Fetuses from experimentally exposed heifers were abodtion either by surgical removal following dam euthanasia captive bolt in the third trimester to days postchallenge or as dead, weak, or healthy calves following natural parturition. Detection and identification of previously unrecognized microbial pathogens.

Diagnosis of epizootic bovine abortion in Nevada and identification of the vector.

Widespread inflammatory lesions with a vascular orientation are evident in most tissues. Epizootiologic and immunologic studies of epizootic bovine abortion. Studies on the pathogenesis of epizootic bovine abortion. Vaccination of cattle against epizootic bovine abortion. Affected fetuses also have markedly elevated immunoglobulin levels, suggestive of a vigorous fetal abogtion response.

Characterization of Pajaroellobacter abortibovis, the etiologic agent of epizootic bovine abortion.

Tissues collected for histopathology formalin included brain, lung, heart, liver, kidney, thymus, lymph nodes, spleen, adrenal, muscle, and digestive tract samples. Epizootic bovine abortion EBAfirst identified in the s, is a major contributor of economic loss to western U. The bivine study employed an shPCR technique in an attempt to identify a bacterial agent present in fetal thymic tissues collected from EBA cases.

Escherichia colia member of the class Gammaproteobacteriawas included as an outgroup in this analysis. While attempts to sequence ten universally conserved protein-coding genes using previously published degenerative primers failed, redesigned primers based solely upon Deltaproteobacteria facilitated the partial sequencing of two genes; fusA JQ and pyrG JQ Second bovvine third repetitions abbortion the shPCR experiments were performed.


Two of the three healthy calves born from antibiotic-treated dams were euthanized immediately prior to consumption of colostrumand tissues were collected for PCR analysis. From the first experiment, sequences corresponding to a novel betaproteobacterium and Ochrobactrum anthropi were identified. A specific PCR was developed described in Materials and Methods to detect this deltaproteobacterium in bovine tissues.

In this study, spizootic PCR was used to identify a fragment of the 16S rRNA gene of a previously undescribed bacterium in thymus tissue derived from affected fetuses. Tissue Presence of agent in fetus a: The focus of the current study was to further expand the morphologic characterization and taxonomic placement of this bacteria, named here as Pajaroellobacter abortibovis.

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Although these agents were detected in some of the DNA samples, the pattern for neither bacterium paralleled the clinical and histopathological diagnosis of EBA data not shown. In addition to identifying this novel agent in diseased fetal bovine tissues, PCR has also demonstrated the presence of the deltaproteobacterium in the tick vector, O. Epizootic bovine abortion EBAsometimes also called foothill abortion, is endemic in California’s coastal range and in the foothill regions of the Sierra Nevada.

In addition, widespread, predominately nonsuppurative histologic lesions typical of EBA were observed in most organs, including the brain, lung, heart, liver, and spleen. In summary, this work demonstrates a close relationship between a novel bacterium and EBA. A specific PCR was subsequently developed to detect the presence of this bacterium in DNA extracted from fetal thymuses.